ISSN : 2146-3123
E-ISSN : 2146-3131

Association Between Superoxide Dismutase Isoenzyme Gene Expression and Total Antioxidant Status in End-Stage Renal Disease Patients on Hemodialysis
Ana Ninić1, Miron Sopić1, Jelena Munjas1, Vesna Spasojević-Kalimanovska1, Jelena Kotur-Stevuljević1, Nataša Bogavac-Stanojević1, Jasmina Ivanišević1, Sanja Simić-Ogrizović2,3, Milica Kravljača2, Zorana Jelić-Ivanović1
1Department for Medical Biochemistry, University of Belgrade, Faculty of Pharmacy, Belgrade, Serbia
2Clinic of Nephrology, Clinical Centre of Serbia, Belgrade, Serbia
3University of Belgrade, Faculty of Medicine, Belgrade, Serbia
DOI : 10.4274/balkanmedj.2018.0170

Background: Chronic renal failure, especially end-stage renal disease is a serious health problem associated with high mortality rate. Uremic syndrome leads to increased oxidative stress, inflammation and dyslipidemia. 
Aims: The purpose of this study was to examine superoxide dismutase isoenzyme gene expression in peripheral blood mononuclear cells of patients on haemodialysis and to determine associations between superoxide dismutase isoenzymes gene expression, oxidative stress and non-enzymatic antioxidative protection. 
Study Design: Case control study. 
Methods: The study included 33 patients on haemodialysis (55.33±15.31 years old) and 33 apparently healthy controls (45.37±8.92 years old). Superoxide dismutase isoenzymes messenger ribonucleic acid levels were determined by Real-time polymerase chain reaction. General biochemical parameters, high sensitivity C-reactive protein, total antioxidant status, thiobarbituric acid-reactive substances and the superoxide anion radical were also determined. 
Results: Normalised Cu/Zn superoxide dismutase and Mn superoxide dismutase messenger ribonucleic acid levels were significantly higher in patients than controls (p<0.001 and p=0.011). There was significant negative correlation between normalised Cu/Zn superoxide dismutase messenger ribonucleic acid levels and total proteins, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and total antioxidant status. Normalised Mn superoxide dismutase messenger ribonucleic acid levels negatively correlated with total proteins and total antioxidant status. Multiple regression analysis revealed independent associations between total antioxidant status and normalised Cu/Zn superoxide dismutase (p=0.038) and between total antioxidant status and normalised Mn superoxide dismutase messenger ribonucleic acid levels (p=0.038 and p=0.018, respectively). 
Conclusion: Superoxide dismutase isoenzyme gene expression in peripheral blood mononuclear cells was higher in patients than in controls. This was probably in response to increased oxidative stress and weakened antioxidative defence. Plasma total antioxidant status was an independent predictive parameter for normalised superoxide dismutase isoenzymes messenger ribonucleic acid levels.

Keywords : Cu/Zn SOD mRNA, Mn SOD mRNA, TAS, Real-time PCR

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