Balkan Medical Journal
Original Articles

Inhibition of YBX1 by miR-216a Suppresses Proliferation and Invasion of Diffuse Large B-Cell Lymphoma


Clinical Laboratory, Liaoning Cancer Hospital & Institute, Shenyang City, Liaoning Province, China


Department of Hematology, Affiliated Hospital of Nantong University, Nantong City, Jiangsu Province, China

Balkan Medical Journal 2021; 38: 171-176
DOI: 10.4274/balkanmedj.galenos.2020.2020.8-23
Read: 208 Downloads: 71 Published: 31 May 2021

Background: MicroRNAs (miRNAs) could be implicated in tumorigenesis of diffuse large B-cell lymphoma (DLBCL).

Aims: To determine the role of MiR-216a in DLBCL.

Study Design: Cell culture study.

Methods: Expression of miR-216a in DLBCL cells was examined by qRT-PCR. Cell counting kit-8, bromodeoxyuridine staining and transwell assays were performed to evaluate role of miR-216a on DLBCL
cell growth. Target gene of miR-216a was verified by luciferase reporter assay.

Results: MiR-216a was dramatically reduced in DLBCL cells compared to the normal B-cell line (P < .01). MiR-216a reduced the viability and retarded DLBCL cell proliferation. The invasion of DLBCL was suppressed by miR-216a. Y box binding protein 1 (YBX1) was validated as a target of miR-216a. Its expression was reduced by miR-216a mimic and enhanced by miR-216a inhibitor in DB and SU-DHL-10 cells. Knockdown of YBX1 reduced cell viability, proliferation, and invasion of DB and SU-DHL-10 cells.

Conclusion: MiR-216a exerted tumor-suppressive effects on DLBCL cells through inhibition of YBX1, providing a new strategy for DLBCL.

Cite this article as: Li Y, Qian J, Yang L. Inhibition of YBX1 by miR-216a suppresses proliferation and invasion of diffuse large B-cell lymphoma. Balkan Med J. 2021; 38(3):171-176.

ISSN 2146-3123 EISSN 2146-3131