ISSN : 2146-3123
E-ISSN : 2146-3131

Selenium Protects Retinal Cells from Cisplatin-Induced Alterations in Carbohydrate Residues
Dilek Akşit 1, Alper Yazıcı 2, Hasan Akşit 3, Esin S. Sarı 2, Arzu Yay 4, Onur Yıldız 3, Adil Kılıç 2, Sıtkı S. Ermiş 2, Kamil Seyrek 5
1Department of Pharmacology and Toxicology, Balıkesir University School of Veterinary, Balıkesir, Turkey
2Department of Ophthalmology, Balıkesir University School of Medicine, Balıkesir, Turkey
3Department of Biochemistry, Balıkesir University School of Veterinary, Balıkesir, Turkey
4Department of Histology and Embryology, University of Erciyes School of Medicine, Kayseri, Turkey
5Department of Medicinal Biochemistry, Balıkesir University School of Medicine, Balıkesir, Turkey
DOI : 10.5152/balkanmedj.2015.155532
Pages : 441-447

Abstract

Background: Investigate alterations in the expression and localization of carbohydrate units in rat retinal cells exposed to cisplatin toxicity.

Aims: The aim of the study was to evaluate putative protective effects of selenium on retinal cells subjected to cisplatin.

Study Design: Animal experiment.

Methods: Eighteen healthy Wistar rats were divided into three equal groups: 1. Control, 2. Cisplatin and 3. Cisplatin+selenium groups. After anesthesia, the right eye of each rat was enucleated.

Results: Histochemically, retinal cells of control groups reacted with α-2,3-bound sialic acid-specific Maackia amurensis lectin (MAA) strongly, while cisplatin reduced the staining intensity for MAA. However, selenium administration alleviated the reducing effect of cisplatin on the binding sites for MAA in retinal cells. The staining intensity for N-acetylgalactosamine (GalNAc residues) specific Griffonia simplicifolia-1 (GSL–1) was relatively slight in control animals and cisplatin reduced this slight staining for GSL-1 further. Selenium administration mitigated the reducing effect of cisplatin on the binding sites for GSL-1. A diffuse staining for N-acetylglucosamine (GlcNAc) specific wheat germ agglutinin (WGA) was observed throughout the retina of the control animals. In particular, cells localized in the inner plexiform and photoreceptor layers are reacted strongly with WGA. Compared to the control animals, binding sites for WGA in the retina of rats given cisplatin were remarkably decreased. However, the retinal cells of rats given selenium reacted strongly with WGA.

Conclusion: Cisplatin reduces α-2,3-bound sialic acid, GlcNAc and GalNAc residues in certain retinal cells. However, selenium alleviates the reducing effect of cisplatin on carbohydrate residues in retinal cells.

Keywords : Cisplatin, n-acetylglucosamine, selenium, sialic acid
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