ISSN : 2146-3123
E-ISSN : 2146-3131

The Role of Follicular Fluid Thiol/Disulphide Homeostasis in Polycystic Ovary Syndrome
Esra Nur Tola1, Nadiye Köroğlu2, Merve Ergin3, Hilmi Baha Oral1, Abdülkadir Turgut4, Özcan Erel5
1In vitro Fertilization Unit, Department of Gynecology and Obstetrics, Süleyman Demirel University School of Medicine, Isparta, Turkey
2Clinic of Gynecology and Obstetrics, University of Health Sciences, Kanuni Sultan Süleyman Training and Research Hospital, İstanbul, Turkey
3Clinic of Biochemistry, Gaziantep 25 Aralık State Hospital, Gaziantep, Turkey
4Department of Gynecology and Obstetrics, Medeniyet University School of Medicine, İstanbul, Turkey
5Department of Biochemistry, Yıldırım Beyazıt University School of Medicine, Ankara, Turkey
DOI : 10.4274/balkanmedj.2017.1140

Background: Oxidative stress is suggested as a potential triggering factor in the etiopathogenesis of Polycystic ovary syndrome related infertility. Thiol/disulphide homeostasis, a recently oxidative stress marker, is one of the antioxidant mechanism in human which have critical roles in folliculogenesis and ovulation.
Aims: The aim of our study is to investigate follicular fluid thiol/disulphide homeostasis in the etiopathogenesis of Polycystic ovary syndrome and to determine its’ association with in vitro fertilization outcome. The study procedures were approved by local ethic committee.
Study Design: Cross sectional design
Methods: Follicular fluid of twenty-two Polycystic ovary syndrome women and twenty ovulatory controls undergoing in vitro fertilization treatment were recruited. Thiol/disulphide homeostasis was analyzed via a novel spectrophotometric method.
Results: Follicular native thiol levels were found to be lower in Polycystic ovary syndrome group than non- Polycystic ovary syndrome group (p=0.041) as well as native thiol/total thiol ratio (p<0.0001). Disulphide level, disulphide/native thiol and disulphide/total thiol ratios were increased in Polycystic ovary syndrome group (p<0.0001). A positive correlation between fertilization rate and native thiol (p=0.01, r=0.53) and total thiol (p=0.01, r=0.052) among Polycystic ovary syndrome patients was found. A positive predictive effect of native thiol on fertilization rate among Polycystic ovary syndrome group was also found (p=0.03, β=0.45, 95% CI=0.031-0.643).
Conclusion: Deterioration in thiol/disulphide homeostasis, especially elevated disulphide levels could be one of the etiopathogenetic mechanism in Polycystic ovary syndrome. Increased native thiol levels is related to fertilization rate among Polycystic ovary syndrome patients and also positive predictor marker of fertilization rate among Polycystic ovary syndrome patients. Improvement of thiol/disulphide homeostasis could be of importance in the treatment of Polycystic ovary syndrome to increase in vitro fertilization success in Polycystic ovary syndrome.

Keywords : Follicular fluid, in vitro fertilization, Polycystic ovary syndrome, thiol/ disulphide homeostasis.
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