ISSN : 2146-3123
E-ISSN : 2146-3131

Alveolar Bone Density Reduction in Rats Caused by Unilateral Nasal Obstruction
Xue Wang1, Yongge Cao2, Zhenhua Liu1, Zihan Wang3, Xiaoying Chu1, Lei Wang4, Xuanxuan Hu1, Han Zhao4, Zhanqiu Diao3, Fengting Peng3, Hui Ye1, Zhensheng Cao3
1Department of Biochemistry, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China
2Haiyuan College, Kunming Medical University, Kunming, Yunnan, China
3School of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China
4School of the Second Clinical Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China
DOI : 10.4274/balkanmedj.galenos.2019.2018.12.11
Pages : 311-319

Abstract

Background: Oral breathing can cause morphological changes in the oral and maxillofacial regions.
Aims: To investigate whether oral breathing affected structural changes in bone tissues.
Study Design: Animal experimentation.
Methods: A total of 48 8-day-old male Sprague−Dawley rats were divided into two groups: a breathing group and a sham (control) group. All Sprague−Dawley rats were killed at 7 weeks after unilateral nostril obstruction modeling. Then, structural changes in bone tissues were detected by micro-computed tomography, and the expression levels of receptor activator of nuclear factor-κB, osteoprotegerin, and receptor activator of nuclear factor-κB ligand in the signal pathway of bone metabolism within the local alveolar bone and serum of rats were detected by reverse transcription-quantitative polymerase chain reaction and Western blotting.
Results: The results showed that receptor activator of nuclear factor-κB ligand and receptor activator of nuclear factor-κB levels in bone tissues and serum in the oral breathing group were higher than those in the control group [Maxillary alveolar bone: receptor activator of nuclear factor-κB ligand (pRNA=0.009, pprotein=0.008), receptor activator of nuclear factor-κB (pRNA=0.008, pprotein=0.009); Mandibular alveolar bone: receptor activator of nuclear factor-κB ligand (pRNA=0.047, pprotein=0.042), receptor activator of nuclear factor-κB (pRNA=0.041, pprotein=0.007); Serum: receptor activator of nuclear factor-κB ligand (pRNA<0.001, pprotein<0.001), receptor activator of nuclear factor-κB (pRNA<0.001, pprotein<0.001)], along with decreased osteoprotegerin expression (Maxillary alveolar bone: pRNA=0.038, pprotein=0.048; Mandibular alveolar bone: pRNA<0.001, pprotein<0.001; Serum: pRNA=0.009, pprotein=0.006) and elevated receptor activator of nuclear factor-κB ligand/osteoprotegerin. Micro-computed tomography analysis indicated a significant difference in the level of bone volume fraction, as well as trabecular thickness in maxillary alveolar bone between the experimental and control groups (p=0.049, p=0.047). Meanwhile, trabecular thickness, and cortical thickness levels in mandibular alveolar bone also differed significantly between the experimental and control groups (p=0.043, p=0.024).
Conclusion: Structural changes of the respiratory system affect the alveolar bone structure and unilateral nasal obstruction may lead to a change in regional specific bone density.

Keywords : Bone mineral density, nasal obstruction, rats, receptor activator of nuclear factor-κB, receptor activator of nuclear factor-κB ligand
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