ISSN : 2146-3123
E-ISSN : 2146-3131

Alveolar Bone Density Reduction in Rats Caused by Unilateral Nasal Obstruction
Xue Wang1, Yongge Cao2, Zhenhua Liu1, Zihan Wang3, Xiaoying Chu1, Lei Wang4, Xuanxuan Hu1, Han Zhao4, Zhanqiu Diao3, Fengting Peng3, Hui Ye1, Zhensheng Cao3
1Department of Biochemistry, Wenzhou Medical University School of Basic Medical Sciences, Wenzhou, Zhejiang, China
2Kunming Medical University Haiyuan College, Kunming, Yunnan, China
3Wenzhou Medical University School of Stomatology, Wenzhou, Zhejiang, China
4Wenzhou Medical University School of the Second Clinical Medical Sciences, Wenzhou, Zhejiang, China
DOI : 10.4274/balkanmedj.galenos.2019.2018.12.11

Background: Oral breathing can cause morphological changes in oral and maxillofacial regions.
Aims: This study aimed to investigate whether oral breathing affected structural changes of bone tissues.
Study designAnimal Experimentation.
Methods: A total of 48 8-day-old male Sprague−Dawley (SD) rats were divided into two groups: breathing groups and sham control) groups. All SD rats were killed at 7 weeks after unilateral nostril obstruction modeling. Then the structural changes of bone tissues were detected by micro-CT, and the expression levels of receptor activator of nuclear factor-κB(RANK), osteoprotegerin (OPG), and receptor activator of nuclear factor-κB ligand RANKL in the signal pathway of bone metabolism within local alveolar bone and serum of rats were detected by RT-qPCR and Western blot. 
Results: The results showed RANKL and RANK levels of bone tissues and serum in the oral breathing group were higher than those in the control group(Maxillary alveolar bone (XAB): RANKL (pRNA=0.009, pprotein=0.008), RANK (pRNA=0.008, pprotein=0.009); Mandibular alveolar bone (NAB): RANKL (pRNA=0.047, pprotein=0.042), RANK (pRNA=0.041, pprotein=0.007); Serum: RANKL (pRNA<0.001, pprotein<0.001), RANK (pRNA<0.001, pprotein<0.001)), along with decreased OPG expression(XAB: pRNA=0.038, pprotein=0.048; NAB: pRNA<0.001, pprotein<0.001; Serum: pRNA=0.009, pprotein=0.006)and elevated RANKL/OPG. The micro-CT analysis indicated that significant difference was found between level of BV/TV, as well as Tb.Th. in maxillary alveolar bone of experimental group and control group (p= 0.049, p= 0.047). Meanwhile, Tb.Th. and Cort.Th. levels in mandibular alveolar bone of experimental group also showed significance compared with that in control group (p= 0.043, p= 0.024). 
Conclusion: This study confirmed that structural changes of the respiratory tract affect alveolar bone structure, and the unilateral nostril obstruction may lead to the change of regional specific bone density.

Keywords : Nasal obstruction; Bone mineral density; RANK; RANKL; Signal pathway of bone metabolism
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