ISSN : 2146-3123
E-ISSN : 2146-3131

Inhibition of Lung Cancer Proliferation by Wogonin is Associated with Activation of Apoptosis and Generation of Reactive Oxygen Species
Chengyang Wang1, Chuangcheng Cui1
1Department of Radiology, The Ninth Hospital of Xi’an Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China
DOI : 10.4274/balkanmedj.galenos.2019.2019.7.75
Pages : 29-33

Abstract

Background: Lung cancer has a very high incidence rate and is one of the commonly diagnosed tumors in developed countries.
Aims: To investigate the effect of wogonin on A549 and A427 lung cancer cells and explore the mechanism involved.
Study Design: Cell study.
Methods: The cytotoxicity effect of wogonin on A549 and A427 lung cancer and BEAS-2B cells was assessed by MTT assay. The onset of apoptosis was assessed by flow cytometry using Annexin V FITC/PI staining. Western blotting was used for the determination of changes in apoptotic protein expression.
Results: Wogonin treatment exhibited cytotoxicity effect selectively on A549 and A427 cells without affecting BEAS-2B normal lung cells. The viability of A549 and A427 cells was reduced to 31% and 34%, respectively, on treatment with 50 µM of wogonin; however, there was no significant reduction in BEAS-2B cell viability on treatment with the same concentration of it. Moreover, the percentage of apoptotic A427 cells showed a significant (p<0.049) increase on treatment with wogonin. Furthermore, the treatment led to a marked increase in the activation of caspase 3/8/9 and the generation of reactive oxygen species (ROS) at 72 h in A427 cells. Digital tomosynthesis studies showed a marked reduction in tumor development on treatment with wogonin.
Conclusion: Wogonin treatment specifically exhibits a cytotoxic effect on lung cancer cells and this effect is associated with activation of apoptosis and generation of reactive oxygen species.

Keywords : Apoptosis, caspases, cell study, cytotoxicity, lung cancer, wagonin
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