ISSN : 2146-3123
E-ISSN : 2146-3131

Antinociceptive Action of Moringa peregrina is Mediated by Interaction with α2-Adrenergic Receptor
Sahar M. Jaffal1, Belal O. Al-Najjar2, Manal A. Abbas3, Sawsan A. Oran1
1Department of Biological Sciences, Jordan University School of Science, Amman, Jordan
2Department of Pharmaceutical Sciences, , Al-Ahliyya Amman University School of Pharmacy, Amman, Jordan
3Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Al-Ahliyya Amman University, Amman, Jordan
DOI : 10.4274/balkanmedj.galenos.2020.2019.11.14

Background: Moringa peregrina is an edible, drought resistant tree native to semiarid countries. It is used in folk medicine as painkiller.
Aims: In this study, the antinociceptive effect of the leaf extract of M. peregrina was studied in mice.
Study design: Animal experimentation.
Methods: Thermal (hot plate and tail immersion tests) and chemical (writhing and formalin tests) pain models in male BALB/c mice (8 animals per group) were used to investigate the mechanism of the antinociceptive action of this plant. In addition, the chemical constituents of the extract were identified using LC-MS analysis and the possible active constituents that interact with the receptor were predicted based on molecular docking.
Results: In writhing test, 200 mg/kg M. peregrina extract inhibited abdominal cramps by 55.97 % (p< 0.001). Also, it reduced the time of paw licking in early and late phases of formalin test by 56.8 % and 65.5%, respectively compared to 50.5% and 48.4% inhibition produced by 30 mg/kg diclofenac sodium in early and late phases, respectively (p< 0.05). This effect was abrogated by yohimbine (1 mg/kg, i.p) but not by methysergide (5 mg/kg, i.p) in late phase only, an indication that the action of M. peregrina in formalin test is not mediated by 5-HT2 serotonin receptors but rather via α2-adrenergic receptors. In hot-plate but not tail-immersion test, the high dose (400 mg/kg) of the extract increased the latency time after 30 min of extract administration. Yohimbine antagonized the action of M. peregrina in hot plate test. Based on LC-MS analysis, the major constituents in M. peregrina methanolic extract were chrysoeriol 7-O-diglucoside, lupeol acetate, quercetin and rutin. Depending on molecular docking results, the activity of M. peregrina extract could be due to the binding of chrysoeriol 7-O-diglucoside, quercetin and rutin to α2-adrenergic receptor.
Conclusion: Our results suggest an interaction with α2-adrenergic receptor as a possible mechanism of M. peregrina analgesic action.

Keywords : α2-adrenergic receptor, analgesic, molecular docking, Moringa peregrina
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