ISSN : 2146-3123
E-ISSN : 2146-3131

New Psychoactive Substance 5-MeO-MIPT In vivo Acute Toxicity and Hystotoxicological Study
Yusuf Ali Altuncı1, Melike Aydoğdu2, Eda Açıkgöz3, Ümmü Güven4, Fahriye Duzağa4, Aslı Atasoy5, Nebile Dağlıoğlu5, Serap Annette Akgür2
1Department of Emergency, Ege University School of Medicine, İzmir, Turkey
2Department of Addiction Toxicology, Ege University Institute on Drug Abuse, Toxicology and Pharmaceutical Science, İzmir, Turkey
3Department of Histology and Embryology, Yüzüncü Yıl University School of Medicine, Van, Turkey
4Department of Stem Cell, Ege University Health Science Institute, İzmir, Turkey
5Department of Forensic Science, Çukurova University, Institute on Addiction and Forensic Science, Adana, Turkey
DOI : 10.4274/balkanmedj.galenos.2020.2019.11.68

Background: The hallucinogenic tryptamine analog 5-methoxy-N-methyl-N-isopropyltryptamine (5-MeO-MiPT) causes social problems worldwide. There are several studies on the metabolism; however, not more studies were found in the literature on acute toxicity.
Aims: We report the acute toxicity of 5-MeO-MiPT in mice, followed by quantitative toxicological analysis of blood and organs, hystotoxicological and immunohistochemical analysis of tissues and cells.
Study design:
In vivo experiments were performed using CD1 adult female mice (n=26). Animals were caged in 4 groups randomly.
Method: First group was a control (n=3). Second group was vehicle control (n=3) and injected 150 µL of blank solution (50% dimethyl sulfoxide in saline /0.9% of NaCl). While for acute toxicity experiments, 5-MeO-MiPT was added to a blank solution in order to obtain a dose of 0.27 mg/kg in 150 µL injection (n=10) and the last group were injected 2.7 mg/kg 5-MeO-MiPT in a 150 µL injection (n=10). Quantitative toxicological analysis, hystotoxicological and immunohistochemical analysis were performed.
Results: In the toxicological analysis, 5-MeO-MiPT was found negative in biological samples which were control, vehicle control, and 0.27 mg/kg dose mice groups. 5-MeO-MiPT was found 2.7-13.4 ng/ml in blood, 11-29 ng/g in kidney, 15.2-108.3 ng/g in liver, and 1.5-40.6 ng/g in the brain in 2,7 mg/kg injected group.
In a low dose of the 5-MeO-MiPT liver section, compared with normal tissues, the difference in staining was statistically significant (p<0.0001). In high-dose of 5-MeO-MiPT, H-score showed that the increase in the number of Caspase-3 positive cells was significant compared to the control (p<0.05). In high-dose of 5-MeO-MiPT, intense Caspase-3 immunoreactivity was observed and the increase in the number of Caspase-3 positive cells compared to the control was statistically significant (p<0.05). In brain section, the statistics of the results obtained from the H-score showed that the increase in the number of Caspase-3 positive cells was significant compared to the control (p=0.0183).
In vehicle control liver section, there were few Caspase-8 positive cells characterized by a light brown appearance (p=0.0117). In the high-dose 5-MeO-MiPT group, the numbers of positive cells at low and high doses of 5-MeO-MiPT group were statistically significant compared to the control (p<0.05). In the high-dose 5-MeO-MiPT group, Caspase-8 immunoreactivity was detected in the glomerular structures. Compared to control, the increase in Caspase-8 immunoreactivity was found to be statistically significant (p<0.05).
Conclusion: Low-dose 5-MeO-MiPT did not cause any serious histopathological effects on the liver, kidney, and brain. High doses induce apoptotic cell death through caspase activity.

Keywords : Forensic toxicology, New Psychoactive Substance (NPS), Tryptamine, 5-MeO-MiPT, Moxy
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