ISSN : 2146-3123
E-ISSN : 2146-3131

Assessment of Eyebright (Euphrasia Officinalis L.) Extract Activity in Relation to Human Corneal Cells Using In Vitro Tests
Roman Paduch 1, Anna Woźniak 2, Robert Rejdak 2, Piotr Niedziela 3
1Department of Virology and Immunology, Maria Curie-Sklodowska University Faculty of Biology and Biotechnology, Lublin, Poland
2Department of General Ophthalmology, Medical University of Lublin, Lublin, Poland
3Department of Surgery, District Railway Hospital, Lublin, Poland
DOI : 10.5152/balkanmedj.2014.8377

Abstract

Background: Euphrasia officinalis L. is an herb traditionally used in folk medicine, mainly in the treatment of eye disorders.

Aims: The present study analyzed the activity of three extracts of E. officinalis L. (ethanol, ethyl acetate and heptane) on cultured human corneal epithelial cells (10.014 pRSV-T).

Study Design: In vitro study.

Methods: Toxicity, free radical scavenging activity and the immunomodulatory effects of the extracts were tested using the thiazolyl blue tetrazolium bromide (MTT) or Neutral Red, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ELISA tests, respectively. Moreover, nitric oxide levels and cytoskeleton architecture were analyzed after corneal cell incubation with the plant extracts.

Results: We show that the biological effect depended on both the concentration and the extraction solvent used. Heptane extracts, distinct from those in ethanol and ethyl acetate, were toxic to 10.014 pRSV-T cells at low concentrations (25 μg/mL) and did not demonstrate free radical scavenging effects. All tested extracts decreased pro-inflammatory cytokine expression (IL-1β, IL-6 and TNF-α) and also anti-inflammatory IL-10 expression by human corneal cells when the extracts were added to the cell culture medium for 24 h.

Conclusion: In conclusion, we show that the promising effects of the application of E. officinalis L. preparations as a supplementary therapy for eye disorders are associated with the ethanol and ethyl acetate extracts, not the heptane extract. 

Total Times Cited: 7 (Updated on Sep. 2017- WoS)

Keywords : Cytotoxicity, Euphrasia officinalis extracts, human corneal cells, immunomodulation, reactive oxygen species scavenging effect
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