ISSN : 2146-3123
E-ISSN : 2146-3131

Evaluation of Antimicrobial Durability and Anti-Biofilm Effects in Urinary Catheters Against E. faecalis Clinical Isolates and Reference Strains
Didem Kart 1, Ayşe Semra Kustimur 2, Meral Sağıroğlu 1, Ayşe Kalkancı 2
1Department of Pharmaceutical Microbiology, Hacettepe University School of Medicine, Ankara Turkey
2Department of Medical Microbiology, Gazi University School of Medicine, Ankara, Turkey
DOI : 10.4274/balkanmedj.2016.1853

Summary

Background: Notably Enterococcus faecalis, Escherichia coli, Staphylococcus epidermidis, Pseudomonas aeruginosa and Candida albicans biofilms are major causes of catheter-associated urinary tract infections. Antimicrobial coated or impregnated urinary catheters are seen as possible way to prevent these infections.
Aims: The aims of this study were to determine the biofilm forming ability in 89 E. faecalis isolates from urinary tract infections and to compare some urinary catheters for antimicrobial durability and the inhibition effects of biofilm formation of both different laboratory strains and clinical isolates of E. faecalis.
Study Design: In vitro experimental study.
Methods: The biofilm forming ability of E. faecalis isolates was determined by the crystal violet staining and plate counting methods. For comparison of urinary catheters, biofilms by 45 E. faecalis isolates from the catheter samples of hospitalized patients and five laboratory strains of Escherichia coli ATCC25922, S. epidermidis ATCC35984, P. aeruginosa ATCC27853, E. faecalis ATCC29212 and C. albicans ATCC90028 were formed on the catheters in 24-well tissue culture plate. Scanning electron microscopy analysis was performed for the biofilm observations.
Results: All 89 E. faecalis isolates were found to be biofilm positive. Nitrofurazone impregnated catheters significantly reduced the cell counts of E. faecalis isolates and also completely inhibited P. aeruginosa and S. epidermidis biofilms compared with the others. In respect to reduction of biofilm cell counts, hydrophilic coated catheter was more effective to P. aeruginosa while silver coated catheter was found more effective to S. epidermidis. Nitrofurazone impregnated catheter had the best antimicrobial durability among the catheters.
Conclusion: Urine isolates of E. faecalis had considerable ability in respect to biofilm formation. Nitrofurazone impregnated catheter was the most effective one against to all tested bacteria, however, the effect of hydrophilic or silver coated catheter depends on the species present in.

Keywords: Biofilm, Enterococcus faecalis, gelatinase, urinary catheter, nitrofurazone, silver

Keywords : Biofilm, Enterococcus faecalis, gelatinase, urinary catheter, nitrofurazone, silver