ISSN : 2146-3123
E-ISSN : 2146-3131

mir-19a-3p Functions as an Oncogene by Regulating FBXO32 Expression in Multiple Myeloma
Ying Li1, Song Gao1, Wenjing Xue1, Yanna Ma1, Yuesheng Meng1, Dawei Zhang2
1Department of Hematology, Jinshan Hospital of Fudan University, Jinshan, Shanghai, PR China
2Department of General Surgery, Jinshan Hospital of Fudan University, Jinshan, Shanghai, PR China
DOI : 10.4274/balkanmedj.galenos.2020.2020.3.121

Background: Multiple myeloma (MM) remains a virtually incurable hematologic malignancy, which is featured with the aberrant growth of malignant plasma cells.
Aims: To elucidate the functions of miR-19a-3p in MM.
Study design: experimental study.
Methods: CCK-8 assay was performed to detect cell viability. Flow cytometry was conducted to detect cell apoptosis. Bioinformatics analysis predicted miR-19a-3p associated biological function, pathway, core regulatory network, and target genes. Luciferase reporter assay verified the target sequence of miR-19a-3p regulating FBXO32.
Results: miR-19a-3p is up-regulated in MM cells (P< 0.01) and patients (P< 0.001). Overexpressed miR-19a-3p significantly increased cell viability (P< 0.05) and inhibited cell apoptosis (P< 0.01). FBXO32 is a target gene of miR-19a-3p (P< 0.01). Besides, FBXO32 is downregulated in MM and significantly decreased cell viability (P< 0.05) and promoted cell apoptosis (P< 0.01). FBXO32 significantly rescued the influence of miR-19a-3p inhibiting cell apoptosis (P< 0.05).
Conclusion: miR-19a-3p promoted cell proliferation and inhibited cell apoptosis by degrading target FBXO32 mRNA in MM.Background: Multiple myeloma (MM) remains a virtually incurable hematologic malignancy, which is featured with the aberrant growth of malignant plasma cells. Aims: To elucidate the functions of miR-19a-3p in MM. Study design: experimental study. Methods: CCK-8 assay was performed to detect cell viability. Flow cytometry was conducted to detect cell apoptosis. Bioinformatics analysis predicted miR-19a-3p associated biological function, pathway, core regulatory network, and target genes. Luciferase reporter assay verified the target sequence of miR-19a-3p regulating FBXO32. Results: miR-19a-3p is up-regulated in MM cells (P< 0.01) and patients (P< 0.001). Overexpressed miR-19a-3p significantly increased cell viability (P< 0.05) and inhibited cell apoptosis (P< 0.01). FBXO32 is a target gene of miR-19a-3p (P< 0.01). Besides, FBXO32 is downregulated in MM and significantly decreased cell viability (P< 0.05) and promoted cell apoptosis (P< 0.01). FBXO32 significantly rescued the influence of miR-19a-3p inhibiting cell apoptosis (P< 0.05). Conclusion: miR-19a-3p promoted cell proliferation and inhibited cell apoptosis by degrading target FBXO32 mRNA in MM.

Keywords : FBXO32, miR-19a-3p, multiple myeloma, oncogene

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