ISSN : 2146-3123
E-ISSN : 2146-3131

In Vitro Vasoactive Effects of Levobupivacaine and Ropivacaine on the Isolated Human Umbilical Artery and Vein
Alper Kılıçaslan 1, Ateş Duman 2, Ayşe Saide Şahin 3
1Dr. Faruk Sükan Kadın Doğum ve Çocuk Hastanesi, Anesteziyoloji ve Reanimasyon Kliniği, Konya, Türkiye
2Selçuk Üniversitesi Meram Tıp Fakültesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Konya, Türkiye
3Selçuk Üniversitesi Meram Tıp Fakültesi, Farmakoloji Anabilim Dalı, Konya, Türkiye
DOI : 10.5174/tutfd.2010.03324.3
Pages : 164-168


Objective: In this in vitro study, we investigated the vasoactive effects of levobupivacaine and ropivacaine on vascular smooth muscle derived from human umbilical arteries and veins.

Material and Methods: The strips were mounted in tissue baths at 37°C continuously gassed with 5% CO2 in oxygen for isometric recording of contractile activity on a polygraph. The endothelium of some tissues was mechanically removed to assess the influence of the endothelium on contractility. The strips were precontracted with serotonin (10-6 M 5-HT; n=7). After obtaining the maximal contraction, responses obtained by adding levobupivacaine and ropivacaine (10-9-10-4 M; n=7) cumulatively were recorded. Contractions were expressed as the (% of 5HT maximal response) percentage of 5 HT's maximal response.

Results: Both levobupivacaine and ropivacaine induce a concentrationdependent contraction in the smooth muscle cells of umbilical arteries and veins. Maximum contractile response (Emax) of levobupivacaine (79.2±2.5, 71.1±2.6) was higher than ropivacaine (68.4±2, 36.2±2.8) on both umbilical arteries and veins. There were no statistically significant differences between contraction responses of endothelium-intact and endothelium-denuded tissues.

Conclusion: The results suggest that, in high concentrations, levobupivacaine and ropivacaine may affect umbilical blood flow by contracting the umbilical artery and vein, thus reducing fetal blood flow.

Keywords : Levobupivacaine, ropivacaine, vasoconstriction, umbilical cord

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