ISSN : 2146-3123
E-ISSN : 2146-3131

Molecular Diversity of Drug Resistant Mycobacterium Tuberculosis Strains in Western Turkey
Süheyla Sürücüoğlu 1, Nuri Özkütük 1, Hörü Gazi 1, Rıza Durmaz 2, Selami Günal 2, Can Biçmen 3, Ayşe Özsöz 4
1Department of Medical Microbiology, Faculty of Medicine, Celal Bayar University, Manisa, Turkey
2Department of Medical Microbiology, Faculty of Medicine, İnönü University, Malatya, Turkey
3Department of Medical Microbiology, Dr. Suat Seren Chest Diseases and Surgery Training and Research Hospital, İzmir, Turkey
4Department of Tuberculosis and Chest Diseases, Dr. Suat Seren Chest Diseases and Surgery Training and Research Hospital, İzmir, Turkey
DOI : 10.5152/balkanmedj.2012.012
Pages : 160-165


Objective: The aim of this study was to investigate the molecular diversity and clonal relationship of drug resistant Mycobacterium tuberculosis strains isolated in Western Turkey.

Materials and Methods: A total of 87 strains isolated between 2006 and 2009, eight of which were rifampicin monoresistant and 79 were multidrug resistant, were analyzed with IS6110 RFLP and spoligotyping methods.

Results: The results of spoligotyping showed that 7% of the strains were orphans, and 8% were undefined for family in the SpolDB4 database. Major families of the strains were LAM (38%), T (35%), Haarlem (7%), Beijing (2%), S (2%) and U (1%) families. The clustering rate by spoligotyping was calculated as 75%. The most predominant SIT cluster was SIT41 (29%). According to the results of IS6110 RFLP, 71 different patterns of IS6110 were observed. Low copy number was found in 26% of the strains. When the results of two methods were combined, the final clustering rate was calculated as 26%.

Conclusions: The genotypical distribution of drug resistant tuberculosis isolates in our region indicates genetic diversity and the clustering rate was found low in our region. However, more comprehensive and long-term molecular epidemiological studies are needed to control the drug resistant strains.

Keywords : Mycobacterium tuberculosis, drug resistance, genotyping, epidemiology
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