Positive Mixed lymphocyte Culture Test Result Due to HLA-DP Mismatch

Tülay Kılıçaslan Ayna ¹, Hakan Gürkan ¹, Hilmi Tozkır ², Hayriye Şentürk Çiftçi ², Mehmet Gürtekin ², Emre Tekgündüz ³, Çetin Algüneş ⁴, Mahmut Çarin ⁵

¹Department of Medical Biology, Faculty of Medicine, İstanbul University, İstanbul, Turkey
²İstanbul Üniversitesi İstanbul Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, İstanbul, Türkiye
³Department of Hematology, Haydarpaşa Training Hospital, Gülhane Military Medical School, İstanbul
⁴Trakya Üniversitesi Tıp Fakültesi Tıbbi Biyoloji Anabilim Dalı, Edirne
⁵Departments of Gastroenterohepatology, İstanbul Medical Faculty of İstanbul University, İstanbul

DOI : 10.5174/tutfd.2009.02852.1

Pages : 224-226

Abstract

Graft Versus Host Disease (GVHD) remains a major cause of morbidity and mortality in allogeneic stem cell transplantation (SCT). The MLC assay has been generally accepted as a standard test for determining HLA-D region compatibility. In this study, the MLC test has been carried out on the related recipient-donor couple who were prepared for allogeneic SCT and were found to be matched for HLA-A, -B, -Cw, DR, DQ but not for -DP. The result of the MLC test was positive. We observed that HLA-DP mismatch was responsible for the increased proliferation values in MLC test.