ISSN : 2146-3123
E-ISSN : 2146-3131

Prevalence and Genetic Diversity of Norovirus in Acute Gastroenteritis Cases in Aydın, Turkey
Sevin Kırdar1, Tülin Başara2, İmran Kurt Ömürlü3
1Department of Medical Microbiology, Faculty of Medicine, Aydın Adnan Menderes University, Aydın, Turkey
2Laboratory of Medical Microbiology Training and Research Hospital, Aydın Adnan Menderes University, Aydın, Turkey
3Department of Biostatistics, Faculty of Medicine, Aydın Adnan Menderes University, Aydın, Turkey
DOI : 10.4274/balkanmedj.galenos.2021.2021-8-22

Aims: Noroviruses may cause both epidemic and sporadic acute gastroenteritis (AGE) globally. Thus, this study evaluated the prevalence of norovirus in stool samples of hospitalized patients with AGE in Aydin, Turkey using enzyme-linked immunoassay (ELISA) and real-time reverse transcription-polymerase chain reaction (rRT-PCR) and genotyped positive samples to detect which genotypes have currently circulated.
Methods: This retrospective descriptive study collected 92 stool samples from patients with AGE symptoms from Aydın Adnan Menderes University Hospital from September 2017 to May 2019. The samples were tested using the commercial Third Generation Ridascreen norovirus ELISA and rRT-PCR. Positive samples were genotyped by sequencing of conventional positive RT-PCR products followed by phylogenetic analysis.
Results: Of the 92 samples, 5 (5.4%) using ELISA and 12 (13%) using rRT-PCR tested positive for norovirus. All positive samples were genogroup II (GII). Two norovirus positive samples were genotyped successfully using DNA sequencing of the nested conventional PCR products. One sample (GII/Hu/TR/2019/Aydin25) could be categorized as GII.3 and the other (GII/Hu/TR/2019/Aydin20) as GII.13.
Conclusion: rRT-PCR testing of stool samples is significantly more sensitive than Ridascreen ELISA. Two norovirus positive samples were successfully genotyped. Data from our study provide protocols on how to study the epidemiology of norovirus.

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