ISSN : 2146-3123
E-ISSN : 2146-3131

Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis
Leyla Acar1, Nazan Atalan2, E. Hande Karagedik1, Arzu Ergen1
1Department of Molecular Medicine, İstanbul University Institute of Experimental Medicine, İstanbul, Turkey
2Clinic of Anesthesia and Reanimation, Siyami Ersek Thoracic Cardiovascular Surgery Training and Research Hospital, İstanbul, Turkey
DOI : 10.4274/balkanmedj.2017.0246
Pages : 30-35

Abstract

Background: The humoral system is activated and various cytokines are released due to infections in tissues and traumatic damage. Nuclear factor-kappa B dimers are encoded by nuclear factor-kappa B genes and regulate transcription of several crucial proteins of inflammation such as tumour necrosis factor-alpha.
Aims: To investigate the possible effect of polymorphisms on tumour necrosis factor-alpha serum levels with clinical and prognostic parameters of sepsis by determining the nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A) gene polymorphisms and tumour necrosis factor-alpha serum levels.
Study Design: Case-control study.
Methods: Seventy-two patients with sepsis and 104 healthy controls were included in the study. In order to determine the polymorphisms of nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A), polymerase chain reaction–restriction fragment length polymorphism analysis was performed and serum tumour necrosis factor-alpha levels were determined using an enzyme-linked immunosorbent assay.
Results: We observed no significant differences in tumour necrosis factor-alpha serum levels between the study groups. In the patient group, an increase in the tumour necrosis factor-alpha serum levels in patients carrying the tumour necrosis factor-alpha (-308 G/A) A allele compared to those without the A allele was found to be statistically significant. Additionally, an increase in the tumour necrosis factor-alpha serum levels in patients carrying tumour necrosis factor-alpha (-308 G/A) AA genotype compared with patients carrying the AG or GG genotypes was statistically significant. No significant differences were found in these 2 polymorphisms between the patient and control groups (p>0.05).
Conclusion: Our results showed the AA genotype and the A allele of the tumour necrosis factor-alpha (-308 G/A) polymorphism may be used as a predictor of elevated tumour necrosis factor-alpha levels in patients with sepsis.

Keywords : Sepsis, polymorphism, tumor necrosis factor-alpha, NF-kappa B
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