ISSN : 2146-3123
E-ISSN : 2146-3131

Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis
Leyla Acar1, Nazan Atalan2, E. Hande Karagedik1, Arzu Ergen1
1Department of Molecular Medicine, İstanbul University Institute of Experimental Medicine, İstanbul, Turkey
2Clinic of Anesthesia and Reanimation, Siyami Ersek Thoracic Cardiovascular Surgery Training and Research Hospital, İstanbul, Turkey
DOI : 10.4274/balkanmedj.2017.0246
Background: Humoral system is activated and various cytokines are released due to infections in tissues and traumatic damage. NF-kB dimers are encoded by NF-kB genes and regulate transcription of several crucial proteins of inflammation such as tumour necrosis factor-alpha (TNFα).
Aims: To investigate the possible effect of polymorphisms on TNFα serum levels with clinical and prognostic parameters of sepsis by determining the NF-kB1 -94 ins/del ATTG and TNFα (-308 G/A) gene polymorphisms and TNFα serum levels.
Study Design: This study was designed as a case-control study. Seventy-two patients with sepsis and 104 healthy controls were included in the study.
Methods: In order to determine the polymorphisms of NF-kB1-94 ins/del ATTG and TNFα (-308 G/A), polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) analysis was performed and serum TNFα levels were determined using an enzyme-linked immunosorbent assay (ELISA).
Results: We observed no significant differences in serum TNFα levels between the study groups. But in the patient group, serum TNFα level in patients carrying TNFα (-308 G/A) A allele and statistically significantly increased compared with those without the A allele. Also in the patient group, serum TNFα levels in patients carrying TNFα (-308 G/A) AA genotype statistically significantly increased compared with patients who carrying AG or GG genotypes. No significant differences were found in these two polymorphisms between the patient and control groups (p>0.05).
Conclusions: Our results showed AA genotype and A allele of the TNFα (-308 G/A) polymorphism may be used as a predictor of elevated TNFα levels in patients with sepsis.
Keywords : Inflammation, level, polymorphism, risk, sepsis
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