ISSN : 2146-3123
E-ISSN : 2146-3131

The Effect of Chemerin on Cardiac Parameters and Gene Expressions in Isolated Perfused Rat Heart
Özden Kutlay1, Ziya Kaygısız1, Bilgin Kaygısız2
1Department of Physiology, Eskişehir Osmangazi University School of Medicine, Eskişehir, Turkey
2Department of Pharmacology, Eskişehir Osmangazi University School of Medicine, Eskişehir, Turkey
DOI : 10.4274/balkanmedj.2017.1787

Background: Chemerin is a novel chemoattractant adipokine, expressed in cardiovascular system and its receptor has also been detected in epicardial adipose tissue.
Aims: The objective of this research is to determine the effects of chemerin on the cardiac parameters and gene expressions, in isolated perfused rat heart.
Study Design: Animal experiment.
Methods: The hearts were retrogradely perfused with Langendorff technique to measure cardiac parameters. 10, 100 and 1000 nM doses of chemerin were acute treated to the experimental groups. In another group, 10 μM L-NAME was given for 5 minutes before 1000 nM chemerin administration. Real-time PCR was performed for detecting expression of target genes.
Results: All doses of chemerin significantly decreased LVDP (max 35.33 ∆%, p<0.001), and +dP/dtmax (max 31.3 ∆%, p<0.001), which are the indexes of cardiac contractile force. In addition, chemerin at a dose of 1000 nM reduced coronary flow (max 31 ∆%, p<0.001).  L-NAME antagonized the negative inotropic effect of chemerin on contractility. Chemerin induced 2.16 fold increase of eNOS mRNA and, also, increased cGMP levels (p<0.001) but decreased PI3Kγ gene expression (1.8 fold, p<0.001). Furthermore, all doses of chemerin decreased CaV1.2 gene expression (1.69 fold, p<0.001).
Conclusion: We suggest that acute chemerin treatment induces a negative inotropic action with the involvement of NO pathway, CaV1.2 and PI3Kγ on isolated rat heart.

Keywords : Chemerin, Heart contractility, Cyclic GMP, Endothelial nitric oxide synthase, L-type Ca2+ channel.
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