ISSN : 2146-3123
E-ISSN : 2146-3131

O6-methylguanine-DNA Methyltransferase Promoter Methylation in Patients with Rectal Adenocarcinoma After Chemoradiotherapy Treatment: Clinical Implications
Jaime A. Oliver1,2, Jaime Gómez-Millán3, Jose A. Medina3, Laura Cabeza2,4,5, Gloria Perazzoli2,5, Cristina Jimenez-Luna2, Kevin Doello6, Raúl Ortiz2,4,5
1Center for Cancer Research and Cell Biology, Queen’s University Belfast, Belfast, UK
2Institute of Biopathology and Regenerative Medicine, Center of Biomedical Research, University of Granada, Granada, Spain
3Department of Radiation Oncology, Universitary Hospital Virgen de la Victoria, Málaga, Spain
4Department of Anatomy and Embryology, University of Granada, Granada, Spain
5Biosanitary Institute of Granada (ibs. GRANADA), SAS-Universidad de Granada, Granada, Spain
6Medical Oncology Service, Universitary Hospital Virgen de las Nieves, Granada, Spain
DOI : 10.4274/balkanmedj.galenos.2019.2018.12.93
Pages : 283-286

Abstract

Aims: To analyze the clinical relevance of O6-methylguanine-DNA methyltransferase in rectal adenocarcinoma treated with chemoradiotherapy followed by surgery.
Methods: Tissue samples from 29 rectal adenocarcinoma patients were obtained after chemoradiotherapy. O6-methylguanine-DNA methyltransferase promoter methylation status was established by methylation-specific polymerase chain reaction. O6-methylguanine-DNA methyltransferase protein levels were determined by immunohistochemistry. Clinicopathologic variables, including treatment regression grade, recurrence, lymph node invasion, and stage and differentiation grade of the tumor, were determined.
Results: The O6-methylguanine-DNA methyltransferase gene promoter was methylated in 81.5% of samples. Most patients (88.9%) showed low O6-methylguanine-DNA methyltransferase protein expression. O6-methylguanine-DNA methyltransferase methylation status was not correlated with any of the clinicopathological variables determined in rectal adenocarcinomas selected for chemoradiotherapy.
Conclusion: O6-methylguanine-DNA methyltransferase methylation status is not correlated with clinicopathologic variables examined in rectal adenocarcinoma selected for chemoradiotherapy, although its role as a biomarker awaits further investigation.

Keywords : Chemoradiotherapy, O6-methylguanine-DNA methyltransferase, rectal adenocarcinoma
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