ISSN : 2146-3123
E-ISSN : 2146-3131

The Effect of Autologous Platelet Rich Plasma in the Treatment of Achilles Tendon Ruptures: An Experimental Study on Rabbits
Baran Şen 1, Serkan Güler 1, M. Hasan Tatari 1, Berivan Çeçen 2, Erdem Kumtepe 2, Alper Bağrıyanık 3, Sermin Özkal 4, M. Ali Özcan 5, Hayri Özsan 5, Namık Şanlı 5
1Department of Orthopaedics and Traumatology, Dokuz Eylül University Faculty of Medicine, İzmir, Turkey
2Department of Biomechanics, Dokuz Eylül University Institute of Health Science, İzmir, Turkey
3Department of Histology and Embriology, Dokuz Eylül University Faculty of Medicine, İzmir, Turkey
4Department of Pathology, Dokuz Eylül University Faculty of Medicine, İzmir, Turkey
5Department of Hematology, Dokuz Eylül University Faculty of Medicine, İzmir, Turkey
DOI : 10.5152/balkanmedj.2015.15549

Abstract

Background: Achilles tendon ruptures are characterized by a long recovery period, high re-rupture rate and late return to work. To overcome these difficulties and augment tendon repair, many agents have been used.

Aims: To determine the effect of autologous platelet rich plasma (PRP) in the treatment of Achilles tendon ruptures in rabbits.

Study Design: Animal experimentation.

Methods: The study included 14 New Zealand albino rabbits that were divided randomly into 2 groups, A and B, each containing seven rabbits. On day zero, all 28 Achilles tendons were tenotomized and repaired. In group A, the tendons were injected with PRP post-surgery, whereas those in group B were left untreated. On day 28, the right tendons in both groups were examined histopathologically via both light and electron microscopy, and the left tendons were subjected to biomechanical testing.

Results: The histological and biomechanical findings in both light and electron microscopy in group A were better than those in group B, but the difference was not significant. According to Tang’s scale, the mean value in Group A was 3.57, while it was 3.0 in Group B. The mean value of Group A for the length of collagen bands was 48.09 nm while the mean value of Group B was 46.58 nm (p=0.406). In biomechanical tests, although stiffness values were higher in group A, the difference between groups was not significant. In addition, maximum load values did not differ between groups A and B.

Conclusion: PRP had no effect on the healing process 28 days post-Achilles tendon rupture.

Keywords : Achilles tendon, electron microscopy, platelet-rich plasma, tendon healing, tendon rupture
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