ISSN : 2146-3123
E-ISSN : 2146-3131

Effects of Apigenin on Experimental Ischemia/Reperfusion Injury in the Rat Ovary
Zeynep Soyman1, Sefa Kelekçi2, Veysel Sal3, Osman Şevket4, Nihan Bayındır5, Hafize Uzun6
1Clinic of Obstetrics and Gynecology, University of Health Sciences, İstanbul Training and Research Hospital, İstanbul, Turkey
2Department of Obstetrics and Gynecology, İzmir Katip Çelebi University School of Medicine, İzmir, Turkey
3Department of Obstetrics and Gynecology, İstanbul University Cerrahpaşa School of Medicine, İstanbul, Turkey
4Department of Obstetrics and Gynecology, Bezmialem Vakıf University School of Medicine, İstanbul, Turkey
5Department of Histology and Embryology, Bezmialem Vakıf University School of Medicine, İstanbul, Turkey
6Department of Biochemistry, İstanbul University Cerrahpaşa School of Medicine, İstanbul, Turkey
DOI : 10.4274/balkanmedj.2016.1386

Apigenin is a plant-derived compound belonging to the flavone class, which possess antioxidant, free-radical-scavenging and anti-inflammatory properties. Administration of apigenin has no significant protective effect on ovarian reserve and tissue damage in ovarian ischemia/reperfusion injury

Background: Apigenin is a plant-derived compound belonging to the flavone class, which possess antioxidant, free-radical-scavenging and anti-inflammatory properties.
Aims: To address the effects of apigenin on serum anti-mullerian hormone levels, tissue oxidative stress parameters and histopathological changes in ovarian ischemia/reperfusion injury.
Study Design: Animal experiment.
Methods: Twenty-eight female Wistar albino rats were randomly separated into four sections: Sham operation (group 1), ischemia/reperfusion plus saline (group 2), ischemia/reperfusion plus dimethyl sulfoxide (group 3) and ischemia/reperfusion plus apigenin (group 4). In all ischemia/reperfusion groups, a bilateral adnexal 3-h period of ischemia was performed, followed by 3-h of reperfusion. A single dose of 15 mg/kg apigenin was given intraperitoneally 60 min before reperfusion in group 4. After 3-h of reperfusion, both ovaries were removed, and blood samples were collected. The main outcome measures were serum anti-mullerian hormone levels, ovarian tissue malondialdehyde, total nitric oxide, Cu/Zn superoxide dismutase, catalase and glutathione levels and histopathological damage scores.
Results: The ovarian tissue nitric oxide level was significantly lower, and the glutathione level was significantly higher in group 4 compared with groups 2 and 3. There was no significant difference in anti-mullerian hormone levels among the three ischemia/reperfusion groups. The histopathological damage score was lower in group 4 than in groups 2 and 3 (p>0.05).
Conclusion: Administration of apigenin has no significant protective effect on ovarian reserve and tissue damage in ovarian ischemia/reperfusion injury.

Keywords : Anti-mullerian hormone, apigenin, ischemia/reperfusion injury, ovary, rat
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