ISSN : 2146-3123
E-ISSN : 2146-3131

The Effects of Baicalin on Myoglobinuric Acute Renal Failure in Rats
Özlem Yalçınkaya Yavuz1, Nurettin Aydoğdu1, Ebru Taştekin2, Necdet Süt3
1Department of Physiology, Trakya University School of Medicine, Edirne, Turkey
2Department of Pathology, Trakya University School of Medicine, Edirne, Turkey
3Department of Biostatistics, Trakya University, School of Medicine, Edirne, Turkey
DOI : 10.4274/balkanmedj.2017.0040
Background: Myoglobinuric acute kidney injury (MAKI) is a uremic syndrome which develops due to damage of skeletal muscle. It was demonstrated that free radicals and nitric oxide play an important role in pathogenesis of MAKI. Baicalin has multiple bioactivities including antimicrobial, anti-inflammatory, and antioxidant properties, as it is a potent free radical scavenger.
Aims: In this study, we aimed to investigte the nephroprotective mechanism of Baicalin on MAKI.
Study Design:Animal experimentation.
Methods:In our study, male Sprague Dawley rats were divided into four groups. Control (n=8), Baicalin (n=8), MAKI (n=10) and MAKI+Baicalin (n=10). The rats were deprived of water for 24 hours before receiving intramuscular injection. The control and Baicalin groups were injected with saline intramuscularly (8ml/kg), the MAKI and MAKI+Baicalin groups were given 50% glycerol 8 ml/kg. One hour later, the control and MAKI groups received saline intraperitoneally (ip), and the Baicalin and MAKI+Baicalin groups were given 200 mg/kg Baicalin. Twenty-four hours after the glycerol injection, urine, blood samples were taken and the kidneys of the rats were harvested under ip injections of anaesthesia.
Results:We found that the levels of creatinine, urea, nitric oxide (NO), alanine transaminase, aspartate aminotransferase, and creatine kinase in serum samples, malondialdehyde, NO, inducible NO synthase (iNOS), endothelial NO synthase (eNOS) concentrations in renal tissue, were increased in the MAKI group compared with the control group (p<0,05). The kidney NO levels and glutathione levels were significantly decreased in the MAKI+Baicalin group compared with the MAKI group (p<0,05). There were no significant differences between any other parameters.
Conclusion:Our results did not show any protective effect of baicalin on MAKI. It may also be due to the fact that the effective factors in the pathogenesis of experimental MAKI differ from other experimental models. Moreover, detailed studies are needed to clarify the effects of baicalin in different doses and treatment durations in glycerol-induced acute kidney injury model.
Keywords : Baicalin, Myoglobinuric acute renal failure, Acute kidney injury, Free radicals, Nitric oxide, Nitric oxide synthase.
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