ISSN : 2146-3123
E-ISSN : 2146-3131

Caffeine Increases Apolipoprotein A-1 and Paraoxonase-1 but not Paraoxonase-3 Protein Levels in Human-Derived Liver (HepG2) Cells
Gülben Sayılan Özgün1, Eray Özgün1, Kıymet Tabakçıoğlu2, Selma Süer Gökmen1, Sevgi Eskiocak1, Erol Çakır1
1Department of Medical Biochemistry, Trakya University School of Medicine, Edirne, Turkey
2Department of Medical Biology, Trakya University School of Medicine, Edirne, Turkey
DOI : 10.4274/balkanmedj.2016.1217

Background: Apolipoprotein A-1, paraoxonase-1 and paraoxonase-3 are antioxidant and anti-atherosclerotic structural high-density lipoprotein proteins that are mainly synthesized by the liver. No study has ever been performed to specifically examine the effects of caffeine on paraoxonase enzymes and on liver apolipoprotein A-1 protein levels.
Aims: To investigate the dose-dependent effects of caffeine on liver apolipoprotein A-1, paraoxonase-1 and paraoxonase-3 protein levels.
Study Design: In vitro experimental study.
Methods: HepG2 cells were incubated with 0 (control), 10, 50 and 200 μM of caffeine for 24 hours. Cell viability was evaluated by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Apolipoprotein A-1, paraoxonase-1 and paraoxonase-3 protein levels were measured by western blotting.
Results: We observed a significant increase on apolipoprotein A-1 and paraoxonase-1 protein levels in the cells incubated with 50 µM of caffeine and a significant increase on paraoxonase-1 protein level in the cells incubated with 200 µM of caffeine.
Conclusion: Our study showed that caffeine does not change paraoxonase-3 protein level, but the higher doses used in our study do cause an increase in both apolipoprotein A-1 and paraoxonase-1 protein levels in liver cells.

Keywords : Caffeine, apolipoprotein A-1, paraoxonase-1, paraoxonase-3, HepG2 cells
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