ISSN : 2146-3123
E-ISSN : 2146-3131

The Effects of Irisin on L-Name Induced Hypertension in Rats
Nurettin Aydoğdu1, Özlem Yalçınkaya Yavuz1, Ebru Taştekin1, Pınar Tayfur1, Oktay Kaya1, Nihayet Kandemir1
1Department of Physiology, Trakya University School of Medicine, Edirne, Turkey
DOI : 10.4274/balkanmedj.galenos.2019.2019.5.113

Background: The cause of about 95% of hypertension, an important public health problem, is unknown. Intensive studies are underway to understand the physiopathology of hypertension. Irisin, a newly discovered hormone, has been reported to dilate vascular smooth muscle and also lower blood pressure acutely.
Aims: We aimed at investigating the effects of chronic irisin treatment on blood pressure and renal functions in a hypertension model established by nitric oxide synthase inhibition by L-NAME treatment.
Study design: Animal experimentation
Methods: Male Sprague Dawley rats were divided into 4 groups (n=8). Control (C) and irisin (I) groups received intravenous saline injection, hypertension (HT) and hypertension+irisin (HT+I) groups received 1.5 mg/100 g L-NAME. L-NAME (150 mg/L) was added to the drinking water of the rats in groups HT and HT+I for 3 weeks. In the second week of the experiment, irisin (50 nmol/day) was given to the rats in groups I and HT+I and saline were administered to the C and HT groups rats for two weeks through subcutaneously placed osmotic minipumps. Blood pressure was measured by tail-cuff plethysmography method. The 24-hour urine, blood and both kidneys of the rats were collected on the 21st day of the experiment.
Results: The HT group had elevated systolic, diastolic and mean arterial blood pressure values compared to the C group, with an decreased reduced glutathione levels in tissue and serum, with an increase in serum oxidized glutathione level (p<0.05). Histopathologically, increased tubular injury, cast formation, glomerular sclerosis and peritubular fibrosis levels were observed (p<0.05). Irisin treatment did not cause any significant change in blood pressure, renal functions and injury scores. However, renal NO levels significantly increased and eNOS immunoreactivity was determined to be reduced (p<0.05). 
Conclusion: In this study, it was seen that chronic irisin treatment at physiological dose did not decrease blood pressure in experimental HT model. In different experimental models of hypertension, the effects of irisin administration at different doses and periods should be investigated extensively.

Keywords : Hypertension, Irisin, Kidney, Nitric Oxide, Oxidative Stress
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