ISSN : 2146-3123
E-ISSN : 2146-3131

GDF9 and BMP15 Expressions and Fine Structure Changes during Folliculogenesis in Polycystic Ovary Syndrome
Meryem İlkay Karagül1, Savaş Aktaş1, Banu Coşkun Yılmaz1, Mustafa Yılmaz2, Gülhan Örekiçi Temel3
1Department of Histology and Embryology, Mersin University School of Medicine, Mersin, Turkey
2Department of Histology and Embryology, University of Health Sciences, School of Medicine, İstanbul, Turkey
3Department of Biostatistics and Medical Informatics, Mersin University School of Medicine, Mersin, Turkey
DOI : 10.4274/balkanmedj.2016.1110


Background: Polycystic Ovary Syndrome (PCOS) is the most frequently seen endocrine disorder in women of the reproductive age with the prevalence of about 10%. Aims: To investigate the efficiency of the growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) during folliculogenesis in a dehydroepiandrosterone (DHEA)-induced mouse PCOS model.
Study Design: Animal experimentation.
Methods: Mice were divided into three groups; control, vehicle and PCOS group. PCOS model mice was developed by the injection of DHEA dissolved in 0.1 ml sesame oil. Ovarian tissues were examined for GDF9 and BMP15 immunofluorescent labeling and electron microscopic examinations.
Results: The immunoreactivity of GDF9 and BMP15 proteins decreased (p<0.05) in PCOS group (27.73±8.43 and 24.85±7.03, respectively) compared with control group (33.72±11.22 and 31.12±11.05, respectively) and vehicle group (33.95±10.75 and 29.99±10.72, respectively). Apoptotic changes were observed in granulosa cells, lipid vacuoles increased in theca cells, there was a thickening and irregularities in basal lamina of granulosa cells, increased electron density in zona pellucida (ZP) in some of the multilaminar primary and secondary follicles in the PCOS model, at the ultrastructural level.
Conclusion: These results suggest that the decrease in GDF9 and BMP15 expressions starting from the primary follicle stage effect the follicle development and the ZP structure and it may cause subfertility or infertility in PCOS.

Keywords : Polycystic ovary syndrome, folliculogenesis, growth differentiation factor 9, bone morphogenetic protein 15