ISSN : 2146-3123
E-ISSN : 2146-3131

GDF9 and BMP15 Expressions and Fine Structure Changes During Folliculogenesis in Polycystic Ovary Syndrome
Meryem İlkay Karagül1, Savaş Aktaş1, Banu Coşkun Yılmaz1, Mustafa Yılmaz2, Gülhan Örekiçi Temel3
1Department of Histology and Embryology, Mersin University School of Medicine, Mersin, Turkey
2Department of Histology and Embryology, University of Health Sciences, School of Medicine, İstanbul, Turkey
3Department of Biostatistics and Medical Informatics, Mersin University School of Medicine, Mersin, Turkey
DOI : 10.4274/balkanmedj.2016.1110

Abstract

Background: Polycystic ovary syndrome is the most frequently seen endocrine disorder in women of reproductive age with a prevalence of about 10%.
Aims: To investigate the efficiency of growth differentiation factor 9 and bone morphogenetic protein 15 during folliculogenesis in a dehydroepiandrosterone-induced mouse Polycystic ovary syndrome model.
Study Design: Animal experimentation.
Methods: Mice were divided into 3 groups: control, vehicle and Polycystic ovary syndrome. Polycystic ovary syndrome model mice were developed by the injection of dehydroepiandrosterone dissolved in 0.1 mL of sesame oil. Ovarian tissues were examined for growth differentiation factor 9 and bone morphogenetic protein 15 using immunofluorescent labelling and electron microscopic examinations.
Results: The immunoreactivity of growth differentiation factor 9 and bone morphogenetic protein 15 proteins decreased (p<0.05) in the Polycystic ovary syndrome group (27.73±8.43 and 24.85±7.03, respectively) compared with the control group (33.72±11.22 and 31.12±11.05, respectively) and vehicle group (33.95±10.75 and 29.99±10.72, respectively). Apoptotic changes were observed in granulosa cells, lipid vacuoles increased in Theca cells and thickening and irregularities were noted in the basal lamina of granulosa cells. An increased electron density in the zona pellucida in some of the multilaminar primary and secondary follicles in the Polycystic ovary syndrome model was also observed at the ultrastructural level.
Conclusion: These results suggest that the decrease in the growth differentiation factor 9 and bone morphogenetic protein 15 expression initiated at the primary follicle stage effect the follicle development and zona pellucida structure and may cause subfertility or infertility in Polycystic ovary syndrome.

Keywords : Polycystic ovary syndrome, folliculogenesis, growth differentiation factor 9, bone morphogenetic protein 15
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