ISSN : 2146-3123
E-ISSN : 2146-3131

In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma gondii
Melek Bilgin 1, Tuba Yıldırım 2, Tuba Yıldırım 2, Murat Hökelek 3
1Microbiology Laboratory, Samsun Education and Research Hospital, Samsun, Turkey
2Department of Biology, Faculty of Arts and Sciences, Amasya University, Amasya, Turkey
3Department of Medical Microbiology, Cerrahpaşa Faculty of Medicine, İstanbul University, İstanbul, Turkey
DOI : 10.5152/balkanmedj.2012.098
Pages : 19-22

Abstract

Objective: Ivermectin and sulphadiazine were tested individually to determine their in vitro effects on Toxoplasma gondii grown in human epidermoid larynx carcinoma (Hep-2) cell culture.

Study Design: In-vitro study.

Material and Methods: Toxoplasma growth was quantities by an enzyme immunoassay performed directly on the fixed cultures, using a rabbit anti-T. gondii immunoglobulin G as the first antibody and a phosphatase-labeled anti-rabbit immunoglobulin G as the second antibody. For each drug, regression models were used to quantify the relationship between optical density values and antimicrobial agent concentrations in the cultures.

Results: The 50% inhibitory concentrations (IC50) of ivermectin and sulphadiazine were found to be 0.2 μg/mL and 7.3 μg/mL after 48 h of exposure, respectively. None of the concentrations tested for each drugs demonstrated toxicity to Hep-2 cells after 72 h of incubation.

Conclusion: These results indicate that ivermectin significantly inhibited replication of the tachyzoites of T. gondii RH strain.

Keywords : Toxoplasma gondii, ivermectin, sulphadiazine, in vitro, Hep-2

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