ISSN : 2146-3123
E-ISSN : 2146-3131

Matrix Metalloproteinase-9 Level and Gene Polymorphism in Sleep Disordered Breathing Patients with or without Cardiovascular Disorders
Meral Yüksel 1, Ayliz Velioğlu Öğünç 1, Hacer Kuzu Okur 2, Zerrin Pelin 3
1Deparment of Medical Laboratory Technicianship, Vocational School of Health Related Professions, Marmara University, İstanbul, Turkey
2Sleep Disorders Unit, Chest Diseases Clinics, Süreyyapaşa Research and Education Hospital, İstanbul, Turkey
3Vocational School of Health Related Professions, Gazikent Hasan Kalyoncu University, Gaziantep, Turkey
DOI : 10.5152/balkanmedj.2012.068
Pages : 8-12


Objective: Obstructive sleep apnea syndrome (OSAS) is associated with increased cardiovascular morbidity and mortality. We aimed to investigate the matrix metalloproteinase-9 (MMP-9) level and MMP-9 gene polymorphism in sleep apnea patients with or without cardiovascular disease.

Study Design: Case-control study. Material and Methods: Two hundred nine patients [Mean age (±SD), 47 (±12) yrs; M/F, 170/39] diagnosed with sleep-disordered breathing were included in the study. Serum MMP-9 level was performed using enzyme-linked immunosorbant assay (ELISA) and MMP-9 gene polymorphism with polymerase chain reaction-restriction fragment length polymorphism. We divided the patient group into two subgroups: (1) patients with confirmed cardiovascular disease, i.e. CV-P Group and (2) patients without cardiovascular disease, CV-N Group. We compared all parameters between the two groups.

Results: There were 56 OSAS patients with cardiovascular disorder (CV-positive group) and 153 OSAS patients without cardiovascular disorder (CVnegative group). CC, CT and TT genotype distributions between groups were similar [31 (55%), 25 (45%), 0 (0%) vs 88 (57%), 61 (40%), 4 (3%); respectively, p>0.05]. MMP-9 level was significantly higher in CV-P patients (442.7±139.3 pg/mL) than in CV-N patients (364.4±165.0 pg/mL; p=0.0018).

Conclusion: Our results showed that the presence of MMP-9 polymorphism was not associated with cardiovascular disease. MMP-9 level was higher in OSAS patients with cardiovascular disorders than without cardiovascular disorders. Finally, MMP-9 genotype was not associated with serum MMP-9 levels.

Keywords : Cardiovascular disease, MMP-9, polymorphism, sleep apnea, hypertension
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